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O seq\u00fcenciador MiSeq \u00e9 uma plataforma de experimenta\u00e7\u00e3o com diversas aplica\u00e7\u00f5es, em especial sequenciamento de genomas pequenos, amplicons, metagenomica, cDNA, dentre outros. A tecnologia principal consiste na amplifica\u00e7\u00e3o da mol\u00e9cula de DNA em fase s\u00f3lida (microc\u00e9lulas) e posterior sequenciamento por fotodigitaliza\u00e7\u00e3o de cada processo de incorpora\u00e7\u00e3o de bases ao longo da s\u00edntese das mol\u00e9culas de DNA. <\/p>\n
A capacidade total de seq\u00fcenciamento de DNA do equipamento \u00e9 de 15 Gb, distribu\u00eddas em at\u00e9 25 milh\u00f5es de reads que representam seq\u00fc\u00eancias de at\u00e9 300 pb de apenas uma ou ambas as extremidades dos fragmentos de DNA. Os kits de prepara\u00e7\u00e3o de amostras comercializados possibilitam multiplexagem de diversas amostras por seq\u00fcenciamento.<\/p>\n
\/*! elementor – v3.5.6 – 28-02-2022 *\/ O laborat\u00f3rio conta com os seguintes equipamentos:<\/p>\n \u00c9 um sequenciador de nova gera\u00e7\u00e3o baseado na t\u00e9cnica de sequenciamento massivo em paralelo <\/p>\n <\/a><\/p>\n \u00c9 um sequenciador de nova gera\u00e7\u00e3o baseado na t\u00e9cnica de sequenciamento massivo em paralelo por leitura de pH durante a incorpora\u00e7\u00e3o de bases na s\u00edntese da fita complementar. \u00c9 utilizado para a fragmenta\u00e7\u00e3o de \u00e1cidos nucl\u00e9icos e ou complexos biol\u00f3gicos como cromatina, liga\u00e7\u00f5es entre prote\u00ednas, complexos DNA-prote\u00edna, dentre outros. \/*! elementor – v3.5.6 – 28-02-2022 *\/ .elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px} MiSeq\u2122 (Illumina Inc.) MiSeq\u2122 \/*! elementor – v3.5.6 – 28-02-2022 *\/ .elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=”.svg”]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block} O seq\u00fcenciador MiSeq \u00e9 uma plataforma de experimenta\u00e7\u00e3o com diversas aplica\u00e7\u00f5es, em especial sequenciamento de genomas pequenos, amplicons, metagenomica, cDNA, dentre outros. A tecnologia principal […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":538,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false},"_links":{"self":[{"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/pages\/640"}],"collection":[{"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/comments?post=640"}],"version-history":[{"count":52,"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/pages\/640\/revisions"}],"predecessor-version":[{"id":993,"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/pages\/640\/revisions\/993"}],"up":[{"embeddable":true,"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/pages\/538"}],"wp:attachment":[{"href":"https:\/\/sites.icb.ufmg.br\/celam\/labgem\/wp-json\/wp\/v2\/media?parent=640"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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\n<\/a><\/p>\n\n\t\t\t\t\t
\nNormas de uso do MiSeq\t\t\t\t\t<\/a><\/h3>\nTABELA DE PRE\u00c7OS<\/h2>\n
Nossos equipamentos<\/h2>\n
NextSeq1000 Illumina<\/h3>\n
\n<\/a>\t\t\t\t\t\tSaiba mais
\n<\/a><\/p>\nIon-PGM<\/h3>\n
\n<\/a><\/p>\n
\n<\/a>\t\t\t\t\t\tSaiba mais
\n<\/a><\/p>\nBioruptor-Diagenode<\/h3>\n
\n<\/a><\/p>\n
\n<\/a>\t\t\t\t\t\tSaiba mais
\n<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"